A Programmable $25 Thermal Cycler for PCR

Document Type



Published by Wiley in Biochemistry and Molecular Biology Education Volume 31, Issue 2, pages 113-4.
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Publication Source

Biochemistry and Molecular Biology Education


This article describes the construction of a safe, programmable, automatic thermal cycler for PCR that can be easily constructed by persons with basic soldering and mechanical skills for under $25 in parts and a modest computer such as IBM 486, all of which are readily available. The cycler relies on the heating provided by an incandescent light bulb and cooling by simple convection.